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| Author(s): |
Thais Diniz de Souza
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Física Gleb Wataghin |
| Defense date: | 2011-07-11 |
| Examining board members: |
Carlos Manuel Giles Antunez de Mayolo;
Marcelo Baptista de Freitas;
Gabriela Castellano
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| Advisor: | Carlos Manuel Giles Antunez de Mayolo |
| Abstract | |
In this dissertation we have produced in vitro joint and cartilages images by two especific methods of phase contrast imaging: Diffraction enhanced imaging (DEI) method and Propagation method. The DEI setup was implemented at the XRD2 beamline of the Brazilian National Synchrotron Light Laboratory (LNLS) in Campinas. The propagation method setup was implemented at the X-ray applied Crystallography Laboratory (LCARX) using a microfocus source (5 µm), that was installed inside of an experimental hut shielded with lead. The samples used were test objects and specimens of joints and cartilages provided by the group of Dr. William Dias Belangero of School of Medical Sciences (FCM-UNICAMP). In the Crystallography Laboratory was built an experimental station to perform tomography with a microfocus source. At this station pictures were realized by the conventional method and the propagation method. At the same laboratory was realized a study in order to colaborate the FCM group, using clinical cases of metal implants in prosthetic joints, we studied the regeneration of bone tissue after the injury to fixation of the prosthesis, using in vitro samples of injured legs of rats. Finally, tomographic acquisitions of rat and rabbit knees were performed in the conventional method and propagation method. The images obtained by the propagation method of both test objects and biological samples show that there is a better image quality, with high contrast in the edges and visualization of structures that are not visible on conventional radiographs. The same happens with the tomographic reconstructions, showing details of the anatomy of the sample, facilitating the visualization of soft tissue damage. This technique has the advantage of not requiring any optical instrumentation, only exploring the focal size of the X-ray source and the distance between sample and detector. In the LNLS was used the XRD2 beamline with 10,34 keV of energy to perform diffraction enhanced images using the detector Pilatus. The diffraction enhanced images had higher quality because this system uses an analyzer crystal placed after the sample. This crystal serves as a narrow angular slit which resulted in images with gain in contrast as compared to the conventional images methods (AU) | |
| FAPESP's process: | 09/04339-7 - Applications of phase contrast radiography in articular cartilage image. |
| Grantee: | Thaís Diniz de Souza |
| Support Opportunities: | Scholarships in Brazil - Master |