Antinociceptive effect of crotalphine in bone pain induced by Walker 256 tumor cells into femoral cavity of rats.

Author(s)

Vanessa Pacciari Gutierrez

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2013-05-09

Examining board members

Yara Cury; Patrícia Brigatte; Maria Lucia Zaidan Dagli; Gisele Picolo; Sandra Coccuzzo Sampaio Vessoni

• Advisor: Yara Cury

Abstract

The aim of the present work was to standardize a new rat model of bone pain induced by the injection of Walker 256 carcinoma cells into the femoral cavity of rats, and to evaluate the analgesic effect of crotalphine, an analgesic peptide. Radiographic, tomographic and scintigraphic analysis showed the presence of diffuse osteolytic processes, destruction of cortical bone tissue and intense osteogenic activity. Increase of soft tissue adjacent to the bone was also detected, being this phenomenon observed in patients with bone cancer. Hystopathological analysis showed the presence of tumor cells in lungs, spleen and liver, indicating the occurrence of metastasis. Tumor cell inoculation induced the presence of hyperalgesia, allodynia and spontaneous pain. Crotalphine (8 <font face=\"Symbol\">mg/kg, p.o.), administered on day 14, blocked these phenomena. This antinociceptive effect is long-lasting effect (2 days) and mediated by peripheral <font face=\"Symbol\">k and <font face=\"Symbol\">d- opioid receptors. Prolonged treatment with crotalphine (14 days) did not cause the development of tolerance to its antinociceptive effect. (AU)

• FAPESP's process: 07/07547-4 - Antitumoral and antinociceptive effects of crotoxin and CNF 021.03 peptide in a model of bone cancer pain in rats
• Grantee: Vanessa Pacciari Gutierrez
• Support Opportunities: Scholarships in Brazil - Doctorate