Anthropophily and mitochondrial genes polymorphism in Aedes scapularis (Rondani) populations in three hidrografic regions in State of São Paulo

Author(s)

Keilla Miki Kobayashi

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Faculdade de Saúde Pública (FSP/CIR)
• Defense date: 2004-05-25

Examining board members

Margareth de Lara Capurro Guimarães; Maria Helena Matté; Délsio Natal

• Advisor: Oswaldo Paulo Forattini

Abstract

Aedes scapularis, is a synanthropic mosquito species which behavior has been extensively reported in severa I studies in epidemiology and ecology. It is important in Public Health, as this species is abundant in modified environments and shows competence to transmit some arbovirus. In this work, we analyze Ae. scapularis in regard to its anthropophily and genetic polymorphism through molecular markers. There were chosen 3 localities in State of Sao Paulo: metropolitan area of Sao Paulo city (Tiete Valley), Ribeira Valley and Paraiba Valley. Collects were taken every 15 days, around or closer to domiciles, from January 2001 to June 2002. To assess bloodfeeding sources from captured females, molecular markers based on mtDNA were applied. There have been identified 25 sources form the 96 analyzed samples, giving an anthropophily rate of 25%, as found in previous studies. For molecular genetics studies, individuals were analyzed at polymorphism in portions of genes COI (Cytocrhome Oxidasis, subunit I) and ND4 (Nicotinamide Adenine Dinucleotide Dehydrogenase, subunit 4) from mitochondrial genome. All genes were amplified through PCR and sequencied. Polymorphism analysis showed that these populations do not differ among the three geographic regions, as they present high genetic structure with high haplotypic diversity. (AU)