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| Author(s): |
Vanessa Bueris
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2008-07-04 |
| Examining board members: |
Waldir Pereira Elias Junior;
Tania Aparecida Tardelli Gomes do Amaral;
Antonio Fernando Pestana de Castro;
Carla Taddei de Castro Neves;
Osvaldo Augusto Brazil Esteves Sant'Anna
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| Advisor: | Waldir Pereira Elias Junior |
| Abstract | |
Typical EPEC exhibit a localized adherence (LA) pattern in epithelial cells, where tight bacterial clusters are produced. Atypical EPEC produce a localized-like (LAL) adhesion pattern, in which the compact clusters are not formed and that occurs in a later stage of the infection. The aim of this study was to characterize the interaction of LAL-adherent atypical EPEC with the epithelial cell. The kinetics of the A/E lesion, the main feature of EPEC pathology, showed a clear delay in its formation by the atypical strains, probably a consequence of the delay observed in the expression of Intimin, Tir and EspA. The complementation of atypical EPEC with the perABC regulator revealed to be effective in not only advancing the expression of these factors, as well the formation of A/E lesion. No correlation was found between the presence of different adhesins already described in DEC and the LAL pattern. The analysis of the possible interaction of Intimin with epithelial cell receptors showed the presence of a 300 kDa peptide that it seems to interact with this adhesin. (AU) | |