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| Author(s): |
Célio Fernando Figueiredo Angolini
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Química |
| Defense date: | 2014-11-04 |
| Examining board members: |
Anita Jocelyne Marsaioli;
Fabio Augusto;
Luciana Gonzaga de Oliveira;
Cintia Duarte de Freitas Milagre;
Georgiana Feitosa da Cruz
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| Advisor: | Anita Jocelyne Marsaioli |
| Abstract | |
In this work we were able to evaluate the action of microorganisms in naturally biodegraded samples and in biodegraded laboratory assays, by optimizing a protocol that uses fast methods of analysis of the polar fraction by ESI-MS. Which was possible to determine the molecular composition of the compounds for oxygen (O, O2, O3 and O4) and nitrogen classes (N, NO, NO2, NO3) present in petroleum. Allowing to correlate those classes with the biodegradation phenomenon, and the depositional environment for the different studied petroleum samples. Even more information is generated about the origin of biomarkers acids through degradation of the respective hydrocarbons. In addition to the main scope of work, we characterize the petroleum microorganisms by mass spectrometry techniques, showing the importance of developing this MO identification technique by the construction of reference libraries and development of more sophisticated software. Also we synthesized a trideuterated biomarker widely used in studies with petroleum (AU) | |
| FAPESP's process: | 10/06557-9 - Investigating mechanisms of degradation of petroleum. |
| Grantee: | Célio Fernando Figueiredo Angolini |
| Support Opportunities: | Scholarships in Brazil - Doctorate |