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| Author(s): |
Marina Yukari Kubota
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2015-07-01 |
| Examining board members: |
Elizabeth Angelica Leme Martins;
Wagner Quintilio;
Josefa Bezerra da Silva
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| Advisor: | Elizabeth Angelica Leme Martins |
| Abstract | |
Lipopolysaccharides (LPS) of L. interrogans, S. enterica and B. pertussis were extracted and the subfraction monophosphoril lipid A (MPLA) prepared by hydrolysis. The LPS is responsible for immunological response and endotoxicity in mammallians, while MPLA has reduced endotoxic activity, but maintain the immunomodulatory capacity. We studied the adjuvant capacity of LPS and MPLA in formulations with LigA or OmpA antigens against leptospirosis. Tests of immunization and challenge in hamsters confirmed the high immune protection activity of LigA and adjuvant capacity of MPLA of salmonela and LPS of lepstopiras, however formulations did not block the renal colonization. The results suggest that there are differences in the systemic immune protection from the kidney immune protection. (AU) | |
| FAPESP's process: | 13/00939-5 - Evaluation of MPLA as in vaccine formulations against leptospirosis |
| Grantee: | Marina Yukari Kubota |
| Support Opportunities: | Scholarships in Brazil - Master |