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| Author(s): |
Ludmila Valino Garcia
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2010-09-23 |
| Examining board members: |
Marcelo de Franco;
Niels Olsen Saraiva Câmara;
Silvio Luis de Oliveira
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| Advisor: | Marcelo de Franco |
| Abstract | |
Mice selected for high (AIRmax) or low (AIRmin) acute inflammatory response were used in tissue regeneration experiments. It was observed that AIRmax present faster ear tissue regeneration than AIRmin mice, suggesting the involvement of common regulatory loci for both inflammation and ear tissue regeneration phenotypes. In the study we investigated some inflammatory phenotypes and global gene expression profiles in AIRmax and AIRmin mice during the initial phase of the ear tissue regeneration. The histological analyses showed that AIRmax regeneration was complete, and cartilage islands and sebaceous glands were formed in the middle of the regenerated area. AIRmin mice displayed not regeneration. Ear thickness oedema and MPO levels were higher in AIRmax than AIRmin mice (P< 0.001). Global expression analysis showed 794 activated and 528 repressed genes in AIRmax, while 1086 activated and 1145 repressed genes were observed in AIRmin mice 48 hours after injury. AIRmax and AIRmin mice presented up-regulated genes over-represented in inflammatory response, cell adhesion and chemotaxis biological themes (Gene ontology). However, down-modulated genes were significant over-represented for transportation in AIRmax and for taxis, muscle contraction and cycle ubiquitin in AIRmin mice. In the previously QTL regions detected on chromosome 1 differentially-expressed Stat1, Casp8, Hspe1 and Il1r2 genes were found, while Lect1, Fndc3 and Egr3 were detected on chromosome 14. qPCR experiments showed high expression of Il-1<font face=\"Symbol\">b, Il-8rb and Mmp9 in AIRmax and Cxcl2, Tnfa, and Tgfb1 in AIRmin mice. We conclude that AIRmax and AIRmin mice presented several (some on chromosomes 1 and 14) differentially-expressed inflammatory genes which could be involved in the acute inflammatory response and ear tissue regeneration phenotypes. (AU) | |