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| Author(s): |
Luis Guilherme Virgilio Fernandes
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-02-20 |
| Examining board members: |
Ana Lucia Tabet Oller do Nascimento;
Waldir Pereira Elias Junior;
Rita de Cassia Cafe Ferreira
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| Advisor: | Ana Lucia Tabet Oller do Nascimento |
| Abstract | |
Leptospirosis is a zoonosis caused by pathogenic bacteria of genus Leptospira. Annotation of the genome sequences of L. interrogans serovar Copenhageni allows the identification and characterization of proteins potentially involved in the pathogenesis of this bacterium, such as lipoproteins and outer-membrane proteins. The present study characterized three genes, OmpL1, LIC10731 and LIC10645, and one of them, OmpL1, was the most frequent among different species of Leptospira. The recombinant proteins were purified by metal-chelating chromatography. All three recombinant proteins promoted humoral and cellular response after immunization in mice. Binding assays showed that all proteins interact to laminin and plasminogen, and additionally protein OmpL1 binds to fibrinogen and plasma fibronectin. OmpL1 was highly reactive with positive-leptospirosis human sera. The results suggest that the proteins encoded by these genes may play a role in the bacterium pathogenesis. (AU) | |
| FAPESP's process: | 10/12591-5 - Characterization and immunological evaluation of two membrane proteins of Leptospira interrogans obtained in the host Escherichia coli |
| Grantee: | Luis Guilherme Virgílio Fernandes |
| Support Opportunities: | Scholarships in Brazil - Master |