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| Author(s): |
Bruna Cristina Favoretto
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-08-29 |
| Examining board members: |
Eliana Faquim de Lima Mauro;
Niels Olsen Saraiva Câmara;
Alicia Susana Couto;
Maria Notomi Sato;
Ana Cláudia Trócoli Torrecilhas
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| Advisor: | Eliana Faquim de Lima Mauro |
| Abstract | |
High molecular weight components (PI) of Ascaris suum extract exert suppressive effect on the immune response to OVA. PI exert direct effect on DCs, decreasing the T lymphocyte proliferation. This effect is independent of TLR2 and 4 as well as MyD88 molecule. In this work we studied the glycoconjugates in PI and the participation of DC-SIGN and MR, in the modulation of the functional activity of DCs. PI components contain high mannose- and complex-type N-linked oligosaccharides and phosphorylcholine residues. PI components containing N-linked glycans inhibited the DCs maturation induced by LPS. The previous incubation of DCs with mannan, anti-DC-SIGN and anti-MR antibodies abolished the modulatory effect of PI on the DCs maturation. It was also observed that the blockage of DC-SIGN and MR in DCs reversed the inhibitory effect of PI in the in vitro T cells proliferative response. Taking together these results show the involvement of DC-SIGN and MR in the recognition of glycosylated components of PI by DCs and in its modulatory effect. (AU) | |
| FAPESP's process: | 10/10393-1 - Study of the involvement of DC-SIGN and MR receptors in the mechanisms of immunesuppression induced by high molecular weight components of Ascaris suum extract |
| Grantee: | Bruna Cristina Favoretto |
| Support Opportunities: | Scholarships in Brazil - Doctorate |