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| Author(s): |
Francielli Mahnic de Vasconcellos
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-08-29 |
| Examining board members: |
Waldir Pereira Elias Junior;
Tania Aparecida Tardelli Gomes do Amaral;
Carla Taddei de Castro Neves;
Irma Nelly Gutierrez Rivera;
Marcelo Palma Sircili
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| Advisor: | Waldir Pereira Elias Junior |
| Abstract | |
A previous study described a strain of enteropathogenic Escherichia coli (EPEC), serotype O142:H34 (Ec48/66), presenting the aggregative adherence pattern (AA), typical of enteroaggregative E. coli (EAEC), and the eae and aatA genes. The aim of this study was to evaluate the role of fimbriae in the AA pattern of Ec48/66. This strain presented the AA pattern in HeLa, HEp-2, HT-29 and T84 cells, and the ability to cause the A/E lesion in HeLa and HT-29 cells. The presence of the genes comprising the operons of AAF/I fimbriae and E. coli common pilus (ECP) was detected. Both operons were sequenced showing high identity with the operons present in EAEC (agg) and EPEC (ecp). Mutants in the aggC and ecp genes maintained the AA pattern and the ability to cause the A/E lesion in HeLa cells. The aggC mutation significantly reduced the adherence. Phylogenetic analyzes located the strain in the group consisting of EPEC. The Ec48/66 strain is an atypical EPEC expressing the AA pattern, which is partially dependent on a variant of AAF/I, but not on ECP. (AU) | |
| FAPESP's process: | 10/05462-4 - CHARACTERIZATION OF THE ADHESIN RESPONSIBLE FOR THE AGGREGATIVE ADHERENCE OF Escherichia coli OF SEROTYPE O142:H34 |
| Grantee: | Francielli Mahnic de Vasconcellos |
| Support Opportunities: | Scholarships in Brazil - Doctorate |