Detection and analysis of carbamate distribution by DESI mass spectrometry in tissues of experimentally intoxicated rats

Author(s)

Marina Frota de Albuquerque Landi

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD)
• Defense date: 2020-07-13

Examining board members

Paulo César Maiorka; Fernanda Auciello Salvagni; Pedro Henrique Vendramini

• Advisor: Paulo César Maiorka

Abstract

Carbamates are acetylcholinesterase inhibitors with many different formulations and uses. Aldicarb and carbofuran, originally formulated for agricultural use, are frequently employed to cause abortions, homicides, suicides and intoxications of domestic and wild animals. They are known as “small lead” due to its dark granular presentation, are offered to animals with food (bait) in a single quickly lethal dosage. When found alive, these animals present clinical signs related to the inhibition of the acetylcholinestesase enzyme, which stimulates muscarinic and nicotinic receptors, such as diarrhea, vomits, depression and convulsions. The macroscopic and histopathological changes are considered unspecific; such are blood ingurgitation in many organs. The diagnosis is obtained after the analysis of samples taken during necropsy and the most used methods are the thin layer chromatography (TLC), high performance liquid chromatography (HPLC), and the recently validated High-performance liquid chromatography-diode array detection (HPLC-DAD). The method of desorption electrospray ionization mass spectrometry (DESI-MS) can also be used. In this technique, a solvent is directed towards a sample surface to desorb and ionize the material, which will be evaporated and analyzed using Orbitrap Q-Exactive. Lastly, the sample is scanned and the information correlated with the spatial distribution of the chemical specimen. (AU)

• FAPESP's process: 18/04289-9 - Detection and analysis of the carbamate distribution by DESI mass spectometry in formalin fixed and paraffin embedded tissues of intoxicated animals
• Grantee: Marina Frota de Albuquerque Landi
• Support Opportunities: Scholarships in Brazil - Master