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| Author(s): |
Andrea Gil Ferreira de Arruda
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2008-08-28 |
| Examining board members: |
Nancy Starobinas;
Fábio Henrique Kwasniewski;
Sonia Jancar Negro
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| Advisor: | Nancy Starobinas |
| Abstract | |
Lines of mice genetically selected for maximal (AIRmax) or minimal (AIRmin) acute inflammatory reaction (AIR) demonstrated differences in the capacity to infiltrate neutrophil cells. The aim of this work is the characterization of the activity of resident or thioglicollate-induced macrophages in the peritoneal exudates in these mice. After 6h, thioglicollate induces the migration of neutrophils being the maximal macrophage migration achieved at 96h. On both lines, thioglicollate-induced macrophages showed higher phagocytic activity of zymosan particles than resident macrophages. Macrophages from AIRmax mice produced higher response with LPS, than AIRmin cells, regarding the expression of TNF-a, IL-6, IL-12, IL-1b, TREM1, DAP12, and synthesis of H2O2 and NO, which is consistent with the high inflammation selected phenotype of AIRmax mice. We observed that resident cells from AIRmin mice secret higher amounts of IL-10 and TGF-b than AIRmax cells. After thioglicollate stimulus, macrophages from AIRmax mice produced higher levels of the anti-inflammatory cytokines. (AU) | |