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| Author(s): |
Lucas Ferreira de Almeida
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2021-06-18 |
| Examining board members: |
Terezila Machado Coimbra;
Lucila Leico Kagohara Elias;
Guiomar Nascimento Gomes;
Alceu Afonso Jordao Junior
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| Advisor: | Terezila Machado Coimbra |
| Abstract | |
Vitamin D (Vit.D) is involved in cellular proliferation and differentiation and regulation of the renin gene, which are important events of nephrogenesis and quiescence of maintaining renal health in adulthood. This study evaluated the angiogenic mechanisms involved in long term renal disturbances induced by Vit.D deficiency during the renal development persistent in adulthood in rats. First-generation male Hannover offspring from mothers fed either with a control diet (control group, CG) or Vit.D-deficient diet (Vit.D- group) were evaluated. Systolic blood pressure (SBP) was measured monthly during the first 6 months after birth, and blood and urine samples were collected to evaluate renal function. Nitric oxide (NO), angiotensin II (ANGII), parathyroid hormone (PTH), calcium, and Vit.D were measured in serum samples. The kidneys were then removed for morphometric, NO, immunohistochemical, and Western blot studies. We also evaluated the expression of vascular growth factor (VEGF) and angiopoietins 1 and 2 and their receptors in renal tissue since this intrinsic renal axis is responsible for endothelial quiescence. Compared to CG, the Vit.D group presented higher SBP, ANG II plasma levels, renin expression, and AT1 receptor expression levels. Capillary rarefaction was observed, as well as an imbalance between pro- and anti-angiogenic factors. The present findings support the role of Vit.D in the renal development for maintaining the integrity of renal microcirculation. (AU) | |
| FAPESP's process: | 17/07118-8 - Influence of vitamin D on renal development in rats |
| Grantee: | Lucas Ferreira de Almeida |
| Support Opportunities: | Scholarships in Brazil - Doctorate |