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| Author(s): |
Raphael Souza Pavani
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-07-11 |
| Examining board members: |
Maria Carolina Quartim Barbosa Elias Sabbaga;
Carlos Renato Machado;
Luiz Ricardo Orsini Tosi
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| Advisor: | Maria Carolina Quartim Barbosa Elias Sabbaga |
| Abstract | |
The telomeric complex, responsible for genomic integrity and stability , is formed by the interaction of DNA with proteins that are responsible for maintaining and protecting these terminals. The eukaryotic RPA complex comprises a heterotrimer, which fulfills several vital functions in the cell , being a fundamental player in replication , repair and recombination. The absence of homologous proteins that protect telomeres in Trypanosoma cruzi lead us to hypothesize that RPA complex could fulfill this function. Thus, this study aimed to characterize TcRPA-1-telomere interaction. We could verify in vitro and in vivo interaction of RPA - 1 with telomeres in epimastigote and trypomastigote lifeforms. The absence of homologs of proteins that interact with telomeric overhang in trypanosomes, the specific interaction RPA-1- telomere , its presence in non- replicative lifeform as well as structural peculiarities of TcRPA-1, lead us to propose that this protein may be involved in telomere protection in this organism. (AU) | |
| FAPESP's process: | 11/16670-0 - The involvement of RPA complex and ORC1 protein in the replication of chromossomal ends in Trypanosoma cruzi |
| Grantee: | Raphael Souza Pavani |
| Support Opportunities: | Scholarships in Brazil - Master |