Full text
| |
| Author(s): |
Rosane de Oliveira
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-10-16 |
| Examining board members: |
Ana Lucia Tabet Oller do Nascimento;
Tania Aparecida Tardelli Gomes do Amaral;
Rita de Cassia Cafe Ferreira;
Rita de Cássia Ruiz;
Ana Marisa Chudzinski Tavassi
|
| Advisor: | Ana Lucia Tabet Oller do Nascimento |
| Abstract | |
Leptospirosis is worldwide zoonosis caused by pathogenic spirochaetes of the genus Leptospira. In the urban settings, rodents are the most important reservoirs. Since the control of the rodents and sanitation measures are not easily implemented, the development of vaccine is necessary to combat the leptospirosis. Thus, the genes sequences of LIC10258, LIC12880 and LIC12238 were selected by bioinformatics analysis and amplified by PCR methodology from genomic DNA of L. interrogans. Evaluation of the adhesion capacity of the recombinant proteins with extracellular matrix components showed that rLIC10258 interacts to laminin and plasma fibronectin. All recombinant proteins were capable to bind plasminogen and to generate plasmin, showing specific proteolytic activity, whereas that only rLIC12238 was capable to bind fibrinogen. The results obtained suggest that these proteins may play a role in leptospiral pathogenesis. (AU) | |