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| Author(s): |
Layra Lucy Maria Albuquerque da Costa
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2008-10-01 |
| Examining board members: |
Orlando Garcia Ribeiro Filho;
Niels Olsen Saraiva Câmara;
Silvio Luis de Oliveira
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| Advisor: | Orlando Garcia Ribeiro Filho |
| Abstract | |
Mice AIRmax and AIRmin exhibit significant differences in the average number of migrating leukocytes and in the protein content of inflammatory exudate produced by polyacrylamide particles. This higher inflammatory capacity of the AIRmax mice is due to three convergent factors: higher local production of chemotactic factors, increased resistance of locally infiltrated neutrophils to spontaneous apoptosis and larger production of mature neutrophils by the bone marrow. Thus, considering the differential capacity of leukocyte production between AIRmax and AIRmin mice, we are studying comparatively the myelocytic differentiation process in vitro. We verified that the BM cells of AIRmax mice showed higher proliferation levels. In addition by qPCR technique it was verified a larger expression of genes involved in the initial stages of myelopoiesis in the AIRmax BM cultures. The analysis of BM cellular protein content by 2D gel electrophoresis revealed quantitative and qualitative differences between two mouse lines. (AU) | |