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| Author(s): |
Antonio Carlos Pedroso
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2009-08-06 |
| Examining board members: |
Antônio José Piantino Ferreira;
Maria Lucia Zaidan Dagli;
Luiziana Ferreira da Silva;
Idercio Luiz Sinhorini;
Tomomasa Yano
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| Advisor: | Antônio José Piantino Ferreira |
| Abstract | |
The b-glucans are structural polysaccharides from yeast cell wall (Saccharomyces cerevisiae), some cereal grains and fungi. The b-glucans have a glucose molecule carbon linked in sites b-1,3 and they have side chains of glucose residue in sites b-1,6. The b-glucans have a helpful effect as anti-inflammatory, antitumoral, hypoglycemic and hypocholesterolemic activity .The safety of b-glucan water-soluble obtained in this work was demonstrated in vitro, using specific-pathogen-free (SPF) cell culture chicken embryo fibroblast and it was confirmed in vivo after injection in chicken pectoral muscle. The b-glucan is a biological modulator due to its ability to increase the innate immune response. The soluble b-glucan in 240 mg/bird dose, associated to Marek\'s disease vaccine diluents showed immunomodulatory effect in humoral immune response. The IgG plasma levels were detected by ELISA method, and the cellular immune response was evaluated by detection IFN-g, IL-2 and IL-6 in vaccinated chickens with recombinant and live vaccines against Gumboro\'s disease using Real-Time PCR technique. The soluble b-glucan used in this trial demonstrated a powerful immune-adjuvant effect enhancing the cellular and humoral immune response against Bursal disease, when associated to recombinant vaccine and live vaccines. (AU) | |