Anticorpos monoclonais e policlonais para identificação do tobamovírus do mosaico do tomateiro (ToMV) em plantas de Tomate (Lycopersicon esculentum Mill.)

Keila Maria Roncato Duarte

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Author(s):	Keila Maria Roncato Duarte Total Authors: 1
Document type:	Doctoral Thesis
Press:	Piracicaba.
Institution:	Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC)
Defense date:	2000-03-01
Advisor:	Flavio Cesar Almeida Tavares
Abstract
The tomato crops are one of the most important vegetables for the world economy and it is very susceptible to vírus diseases, among them the tomato mosaic tobamovirus (ToMV) that causes light and dark green mosaic in the leaves, decreases the production, among other symptoms. With the aim to early identify ToMV in biological material, harvested from crop fields, monoclonal antibodies (MAbs) and polyclonal antibodies were produced against ToMV. The MAb 10.Hl was chosen by PTA-ELISA (Plate Trapped Antigen-Enzyme-Linked immunoassay) and do not crossreact with TMV (tobacco mosaic tobamovirus) or with proteins extracted from plant sap. The MAb and the polyclonal antibodies produced were able to identify ToMV from infected plants collected in field. These plants were used to isolate the virus, which were purified and used to re-inoculate tobacco and tomato plants to confirm the symptoms. ln immunoblots assays the polyclonal antibodies recognize two protein bands (17.5 kDA and the 30 kDa bands) and the MAb recognizes only the band corresponding to the coat protein of the ToMV (17.5 kDa). The MAb 10.Hl opens the possibility to identify ToMV in tomato seedlings avoiding its dissemination in the crop fields. (AU)

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