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| Author(s): |
Pamela Siumey Liu
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2016-01-28 |
| Examining board members: |
Waldely de Oliveira Dias;
Tania Aparecida Tardelli Gomes do Amaral;
Mickie Takagi
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| Advisor: | Waldely de Oliveira Dias |
| Abstract | |
Transcutaneous immunization (TCI) offers an attractive pathway for the development of needle-free vaccination by acting on APCs of the skin, showing potential to replace conventional immunization, in terms of safety and efficacy. In this study we evaluated the immune response by TCI with recombinant protein of leptospira LipL32, a highly conserved protein among pathogenic strains, a potential vaccine candidate. TCI with LipL32 was evaluated in C57BL-6 mice abdominal region and was able to confer systemic response with high levels of antibodies after subimmunizing ID and Tc boosters. The pattern of cytokines in cell cultures from whole blood of immunized groups indicated that the TCI was able to prime the immune system, for both innate and adaptive response. Local treatment of the skin or coadministration with surfactants and PEG did not show an emollient and an adjuvant action. Co-administration of LipL32 with MPL-A influenced the antibody response as well as showed a moderating effect of the pro-inflammatory reaction, redirecting the adaptive response. (AU) | |
| FAPESP's process: | 12/25176-1 - Immune response induced in mice by transcutaneous immunization with recombinant leptospira Lip L32 protein |
| Grantee: | Pamela Siumey Liu |
| Support Opportunities: | Scholarships in Brazil - Master |