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| Author(s): |
Mara Adriana Corrêa
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2015-02-12 |
| Examining board members: |
Marcelo de Franco;
Gustavo Pompermaier Garlet;
Lourdes Isaac;
Eliana Faquim de Lima Mauro;
Silvio Luis de Oliveira
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| Advisor: | Marcelo de Franco |
| Abstract | |
Pristane-induced arthritis (PIA) in AIRmax mice homozygous for Slc11a1 R and S allele was used in this study to characterize the role of Slc11a1 polymorphisms on immune response, more specifically in the activation of peritoneal macrophages during PIA. Previous reports showed the presence of S allele of Slc11a1 increased the incidence and severity PIA in AIRmaxSS, suggesting that this gene or another closed-linked gene interacts with inflammatory loci to modulate PIA. Pristane treatment induced intense infiltration of lymphocytes, monocytes/macrophages and neutrophils in AIRmaxSS animals. AIRmaxSS macrophages demonstrated exacerbated cellular and gene expression profiles during PIA, with higher expression/production of H2O2, NO, IL-1b, IL-6, TNF-a and chemokines. However, Slc11a1 R allele could be regulating macrophage activation intensity more efficiently than the S allele and control the development of arthritis. There was involvement of kidney, lung and thymus during PIA. Our data suggest that the Slc11a1 gene modulates macrophage activation involved in PIA susceptibility and these lines represent an alternative murine model of rheumatoid arthritis. (AU) | |
| FAPESP's process: | 09/18414-0 - THE GENE Slc11a1 PARTICIPATION IN THE MODULATION OF THE IMMUNE RESPONSE ON PRISTANE-INDUCED ARTHRITIS IN GENETICALLY SELECTED MICE FOR THE ACUTE INFLAMMATORY RESPONSE |
| Grantee: | Mara Adriana Corrêa Giacoia |
| Support Opportunities: | Scholarships in Brazil - Doctorate |