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| Author(s): |
Daniela Tiemi Myamoto
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2015-09-14 |
| Examining board members: |
Denise Vilarinho Tambourgi;
Patricia Antonia Estima Abreu de Aniz;
Inácio de Loiola Meirelles Junqueira de Azevedo;
Ângela Silva Barbosa;
Lourdes Isaac
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| Advisor: | Denise Vilarinho Tambourgi |
| Abstract | |
The complement system seems to have arisen with the appearance of C3 and factor B (FB), the only components found in the most primitive organisms, suggesting that the alternative pathway is the oldest. cDNA fragments coding for the complement factor B (Lox-FB) and C3 (Lox-C3) were synthesized from total RNA Loxosceles laeta venom gland and amplified using RACE-PCR techniques. Lox-FB has a classical domain organization in mosaic, composed by CCPs, vWFA and serine protease domains. The amino acids involved in binding to C3b are conserved, however, the classical calatytic triad was not found. Lox-C3 presents a similar configuration of domains to C3 human and has two putative processing sites: the first one is located between α and γ chains and other between α and β chains, indicating that Lox-C3 is composed by three chains. The phylogenetic analyses indicated that Lox-C3 and Lox-FB are evolutionary closer to the equivalent components of Hasarius adansoni, with identity values of 53% and 43%, respectively. (AU) | |
| FAPESP's process: | 12/03308-3 - Characterization and phylogenetic analysis of genes coding for C3 and factor B complement system components from Loxosceles spiders |
| Grantee: | Daniela Tiemi Myamoto |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |