Full text
| |
| Author(s): |
Juliana Barbosa Casals
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2011-07-08 |
| Examining board members: |
Carlos Eduardo Ambrósio;
Ana Flávia de Carvalho;
Felipe Perecin
|
| Advisor: | Carlos Eduardo Ambrósio |
| Abstract | |
Stroke is the second cause of death worldwide, each year more than 20 million individuals are affected by this disease, 5 million of these die. In Brazil, this is the leading cause of death. Polls show the use of numerous animal models valid in research seeking treatments for stroke functional is increasing. Numerous species are used, rats, rabbits, dogs, pigs, sheep and species of primates. A large number of studies have been done with stem cells from different sources in different animal models of spinal cord injury, and even therapeutic trials in humans, showing promising results. The ideal stem cells for the performance of transplants should be immunologically inert, from sources of easy access, have rapid expansion in culture, ability to provide long-term survival and integration in the host site as well as being suitable for transfection and expression of exogenous genes. The objectives of this research is to establish a model of stroke in rabbits (Oryctolagus cuniculus) to the use of therapy with stem cells and measure the induced lesion and its consequences by fluoroscopic, behavioral assessment, clinical parameters and histological examination. We designed the experiment, the induction of stroke in rabbits, the cell culture of the olfactory epithelium of dogs and rabbits, transduction with retroviruses, the analysis of exogenous gene expression, the preparation of transducted stem cells for transplantation in animals, fluoroscopic, the behavioral assessment, macroscopic evaluation and histology. Thus, the experimental model was validated and the cell implantation in damaged tissue was confirmed. We emphasize that the lack of studies using markers for the tracking of stem cells after transplantation into brain vascular lesions, the possibility of combined use of cell therapy in animals with vascular brain injury from trauma and various diseases in veterinary clinics are facts that justify such a search. So we must reinforce the importance of research on cell therapy to direct and indirect insertions of this biotechnology in human and veterinary medicine. (AU) | |
| FAPESP's process: | 09/03863-4 - Stroke model in rabitts for use in stem cells therapy. |
| Grantee: | Juliana Barbosa Casals |
| Support Opportunities: | Scholarships in Brazil - Master |