Development of protein vaccines against Streptococcus pneumoniae: characterization of the adjuvant components of the cellular pertussis vaccine and analyze of new vaccine combinations.

Author(s)

Carolina Salcedo Rivillas

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2015-06-26

Examining board members

Maria Leonor Sarno de Oliveira; Viviane Fongaro Botosso; Maria Cristina de Cunto Brandileone; Viviane Maimoni Gonçalves; Rita de Cássia Ruiz

• Advisor: Maria Leonor Sarno de Oliveira

Abstract

Previously our group showed that the pneumococcal surface protein A from Streptococcus pneumoniae (pneumococcus), PspA, when combined with cellular pertussis vaccine (wP) as adjuvant, induce high levels of antibodies and mice protection on pneumococcal challenge. In this work were used mutants and purified components derived from B. pertussis for investigate the components responsible for this adjuvant effect, through of mice immunizations in combination with the PspA5 (Clade 5 PspA) antigen. Pertussis toxin (PT) was able to inducing the higher levels of PspA5 specific antibodies and significative protection against the pneumococcal lethal challenge, independently of its enzymatic effect. Was observed that in the protection conferred by the PspA5-wP formulation thus as the anti-PspA5 antibodies, the complements proteins are essentials. Lastly, prime-boost experiments showed that the cellular vaccines BCG and DTP when combined to PspA5, induced high levels of anti-PspA5 antibodies and protection against invasive challenge with pneumococcus. (AU)

• FAPESP's process: 12/10593-6 - Development of protein vaccines against Streptococcus pneumoniae: characterization of the adjuvant components characterization of the cellular pertussis vaccine and analysis ze of new vaccine combinations
• Grantee: Carolina Salcedo Rivillas
• Support Opportunities: Scholarships in Brazil - Doctorate