Molecular detection of Sarcocystidae family in tissues samples of wild rodents (Cavia spp., Ctenomys spp., Myocastor coypus) deposited in museums of Rio Grande do Sul.

Author(s)

Natalia Lopez Orozco

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2013-07-02

Examining board members

Solange Maria Gennari; Luciana Regina Meireles Jaguaribe Ekman; Alda Maria Backx Noronha Madeira

• Advisor: Solange Maria Gennari

Abstract

Coccidia Sarcocystidae are important transmissible agents in human-animal interface. Its diagnosis is difficult due to the availability of samples, without harming the wildlife animals populations. We evaluated, by amplification of ITS-1 the frequency of those coccidia in tissue samples of rodents Cavia spp., Ctenomys spp. Myocastor coypus deposited in museums in Rio Grande do Sul. Of the 75 sampled rodents, DNA of Toxoplasmatinae subfamily was obtained in skeletal muscle (3/69) of M. coypus and Cavia spp. and brain of Cavia spp. (1/30) identified as Toxoplasma gondii. Additionally, Hammondia triffittae was detected in the diaphragm of a M. coypus. The subfamily Sarcocystidae was confirmed in skeletal muscle of Ctenomys spp. (Sarcocystis felis-like) and M. coypus (Sarcocystis spp.). Molecular detection of T. gondii, H. triffittae, Sarcocystis spp. and S. felis-like in three species of Brazilian wild rodents free-living demonstrate their participation in the sylvatic cycle, and potential transmission to humans and other animals. (AU)

• FAPESP's process: 10/14310-3 - Molecular detection of Sarcocystidae parasites in tissue samples of wild rodents (Cavia aperea, Ctenomys spp. Myocastor coypus) deposited in museums of Rio Grande do Sul
• Grantee: Natalia Lopez Orozco
• Support Opportunities: Scholarships in Brazil - Master