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| Author(s): |
Guilherme Gomes Silveira
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2010-05-18 |
| Examining board members: |
Gil Benard;
Jose Alexandre Marzagao Barbuto;
Jorge Simão do Rosário Casseb
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| Advisor: | Gil Benard |
| Abstract | |
Despite more than 20 years of effort, the design of an effective HIV-1 vaccine remains an enormous challenge. In this scenario, new immunological approaches must be considered. Monocytes from HIV-serodiscordant couples and non HIV exposed controls were differentiated in vitro into dendritic cells (MoDC), pulsed with different virus antigens and cultured with autologous lymphocytes. The T lymphocyte immunological response and the MoDC phenotype and function were determined. MoDCs were shown to be fully matured and activated in all antigen-pulsing protocols. Lymphocytes stimulated by DC pulsed with inactivated HIV or p55Gag protein showed greater activation, proliferation and IFN<font face=\"Symbol\">γ production in comparison to those stimulated by non pulsed MoDC. Nonetheless, T cells from non-exposed controls elicited the same response, except for the IFN<font face=\"Symbol\">γ production. This MoDC vaccine model may represent a viable and promising alternative of therapeutic vaccination against HIV. (AU) | |