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| Author(s): |
Matheus Pereira Postigo
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Carlos. |
| Institution: | Universidade de São Paulo (USP). Instituto de Química de São Carlos (IQSC/BT) |
| Defense date: | 2012-05-11 |
| Examining board members: |
Luiz Alberto Colnago;
Rodrigo Bagueira de Vasconcellos Azeredo;
Edenir Rodrigues Pereira Filho;
Otavio Henrique Thiemann
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| Advisor: | Luiz Alberto Colnago |
| Abstract | |
The biochemical research in the field of Metabolomics/ Metabonomics has grown considerably in recent years because its capability of acquiring a large amount of information about the behavior of an organism through its metabolism. For this, it often applies several analytical techniques such as Nuclear Magnetic Resonance. Ivermectin is a drug widely used in Brazil, for its effectiveness in controlling verminosis and pests in livestock (and humans) and is here inserted in the metabolomic/metabonomic context because of the numerous breaches occurred in brazilian beef exports. Failures to comply with the appropriate withdrawal periods for slaughtering treated animals may reflect seriously on the quality of these products. Thus, we used Ivermectin as a metabolism change inducer in cattle and mice, trying to correlate these variations to the applied dose. Through auxiliary tools such as 2D-NMR and chemometric exploratory tools, we evaluated samples of bovine blood plasma and urine, and blood plasma of Balb-C mice, after Ivermectin administration. The results show that Ivermectin has influence on the organism\'s energy balance, interfering with lactate and β-hydroxybutyrate which can be connected to the onset of a critical metabolic condition in mammals, related to the high concentration of ketone bodies in their blood stream. (AU) | |
| FAPESP's process: | 08/00003-1 - Metabolomic Analysis using Steady-State Free Precession NMR (SSFP). |
| Grantee: | Matheus Pereira Postigo |
| Support Opportunities: | Scholarships in Brazil - Doctorate |