Caracterização de antígenos de cisticercos e sua aplicação para o diagnóstico da cisticercose humana

Author(s)

Noeli Maria Espindola

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ)
• Defense date: 2004-01-23

Examining board members

Adelaide Jose Vaz; Irene Fernandes; José Antonio Livramento; Luis dos Ramos Machado; Harumi Ando Takehara

• Advisor: Adelaide Jose Vaz

Abstract

Taenia crassiceps cysticerci were kept in vitro during 144 h. Excretory/Secretory (ES) antigens (peptides <30 kDa) were identified in 24 (ES24) and 48 h (ES48). ES peptides 30-, 18-and 14-12- kDa were recognized by polyclonal antibodies produzed in rabbits immunized with Taenia crassiceps (Tcra) and Taenia solium (Tso) antigens, and antibodies in samples of serum and cerebrospinal fluid (CSF) from patients with neurocysticercosi (NC). Monoclonal antibodies (MoAb) were prepared against ES-Tcra, Tcra vesicular fluid (VF-Tcra), Tso vesicular fluid (VF-Tso), Tso total (T-Tso) and Tso scolex (E-Tso) antigens. The MoAb were utilized in characterization of Tcra and Tso antigens, and in the antigens detection in samples of patients with NC. Twenty four MoAb were obtained, from which nine (1 anti-ES-Tra, 1 anti-VF-Tcra, 3 anti-VF-Tso and 4 anti-T-Tso) reacted with peptides 18- and 14-12- kDa in Tcra antigens, and peptide 14-12 kDa in Tso antigens. The MoAb maintained their reactivity towards VF-Tcra antigen treated with heat and alkaline borohydride and showed reduction after trichloroacetic acid and periodate oxidation treatments. Peptides <20 kDa in VF-Tcra antigen were purified by anti-Tcra MoAb and were used to detect antibodies in serum and CSF taken from patients with NC. Anti-Tcra and anti-Tso MoAb with reactivity for Tera and Tso antigens were used to detect antibodies, and anti-Tcra MoAb to detect antigens in serum and CSF from patients with NC. (AU)