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Evaluation of marginal adaptation of restorative techniques in enamel and dentin submitted to erosive and abrasive cycling

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Author(s):
Mirian Lumi Yoshida
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO)
Defense date:
Examining board members:
Ana Cecilia Corrêa Aranha; Taciana Marco Ferraz Caneppele; Patricia Moreira de Freitas Costa e Silva; Paulo Vinicius Soares
Advisor: Ana Cecilia Corrêa Aranha
Abstract

This in vitro study evaluated the marginal adaptation of the tooth-restoration interface in different restorative techniques and materials used to treat noncarious cervical lesions (NCCLs). Class V cavities were prepared on the buccal surface of 80 bovine incisors (5 mm mesio-distal width, 4 mm occlusal-cervical height and 2 mm deep), and specimens were randomly divided into 8 groups (n=10). Groups G1 to G4 were the control groups, without erosive and abrasive cycling, and G5 to G8 were submitted to cycling. All specimens proceeded to the restorative procedure according to the experimental groups: G1 and G5 - direct technique: restored with Filtek Z350 XT composite resin (3M ESPE); G2 and G6 - direct technique: restored with Filtek One Bulk-Fill composite resin (3M ESPE); G3 and G7 - direct-indirect technique: restored with Filtek Z350 XT composite resin (3M ESPE); G4 and G8 - indirect technique: restored with lithium disilicate-reinforced ceramics (Emax, Ivoclar Vivadent). After restorations, all specimens were analyzed by optical coherence tomography (OCT) (T1). Afterwards, the specimens were submitted to thermocycling, followed by the evaluation according to parameters present in the FDI World Dental Federation criteria, modified for an in vitro study. Restorations were evaluated a second time by OCT (T2), immersed in 50% ammoniacal silver nitrate solution and evaluated again by OCT (T3) images. The images of each restoration were measured in micrometers (µm) and scores. After OCT reading, buccal-lingual cuts were performed at the center of the restorations and analyzed by marginal microleakage scores in the stereoscopic magnifying glass. Finally, scanning electron microscopy analysis was performed to qualitatively evaluate the tooth-restoration interface. The results were analyzed by Kruskall-Wallis, Mann-Whitney and Friedman tests, with a significance level of 5% and Tukey post-hoc test. In the analysis by the FDI criteria, there was no statistically significant difference, with all restorations being considered clinically acceptable. In the OCT analysis, there were only statistically significant differences (p<0.05) in the enamel sound substrate, in which the direct techniques presented lower values of microleakage. In the eroded enamel substrate and both dentin substrates, the results showed that all groups presented less gaps in T1, which increased significantly in T2 and T3, not differing from each other. The same occurred in the analysis by marginal microleakage with dye. Scanning electron microscopy analysis showed the presence of gaps in almost all samples and, in general, the adhesive and resin cement showed better adhesion in enamel when compared to dentin. Given the results presented, it can be concluded that in sound substrates, the direct technique presented the best results and indication. However, in the eroded substrate, the best restorative material and technique was not found, since all presented high values of marginal microleakage. The sensitivity of the adhesive restorative technique in non-carious cervical lesions requires planning of its steps and careful execution. (AU)

FAPESP's process: 18/12928-1 - Evaluation of direct, indirect and associated restorative techniques in the marginal sealing of non-carious cervical lesions
Grantee:Mirian Lumi Yoshida
Support Opportunities: Scholarships in Brazil - Master