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| Author(s): |
Diego Dantas Almeida
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2016-12-07 |
| Examining board members: |
Inácio de Loiola Meirelles Junqueira de Azevedo;
Rodrigo da Silva Galhardo;
Nancy Oguiura;
Eduardo Moraes Rego Reis;
Marie Anne van Sluys
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| Advisor: | Inácio de Loiola Meirelles Junqueira de Azevedo |
| Abstract | |
The pit viper Bothrops jararaca is the most medically important snake in Brazil. Several studies were conducted in order to characterize the components of snake venom. However, the molecular basis of snake genomes is poorly known. Hence, it was carried out the sequencing and assembly of the Bothrops jararaca snake genome. Shotgun and mate-pair libraries were constructed to perform sequencing runs using Illumina technology and complementary sequences were obtained in PACBIO RS II equipment. A BAC library was also constructed and 768 pools of 12 BACs were sequenced. A large number of genomic segments was obtained. It was possible to identify genes of several toxins, including SVMPs, SVSPs, BPPs, CRISPs and VEGF. In addition, it was possible to infer the genomic context related to most of these genes and identify the main genomic repetitive elements. These findings are relevant for understanding the function and evolution of the venom system and it provides the basis for further studies. (AU) | |
| FAPESP's process: | 13/07974-0 - Sequencing of Bothrops jararaca genome to characterize toxins gene structure and their regulatory elements |
| Grantee: | Diego Dantas Almeida |
| Support Opportunities: | Scholarships in Brazil - Doctorate |