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| Author(s): |
Luciana de Araujo Pimenta
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2015-11-27 |
| Examining board members: |
Sandra Coccuzzo Sampaio Vessoni;
Sandra Helena Poliselli Farsky;
Ana Paula Lepique
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| Advisor: | Sandra Coccuzzo Sampaio Vessoni |
| Abstract | |
CTX stimulates, in vivo and in vitro, the macrophages secretory capacity in the presence of tumor, accompanied by a significant tumor mass reduction and tumor cells or inhibition of the proliferation, respectively. Considering the immunomodulatory action of CTX, the pivotal importance of macrophages in the genesis, tumor progression and in the stromal microenvironment, the macrophages secretory activity treated with CTX on angiogenesis was investigated in vitro. Thymic endothelial cells (t.End.1), when incubated in the presence of macrophages treated with CTX (0.3g/ml) or supernatants of these macrophages showed significant inhibition of proliferation, adhesion to its natural ligands (Type I collagen-10mg/ml ; fibronectin-3g/ml and laminin-10/mL), and of the cell number migrated in Wound healing model as well as the cell migration velocity, evaluated in Time Lapse. Consequently, there was inhibition of the cappilary-like tube formation on matrigel-3D matrix, accompanied by VEGF and TNF- secretion decrease. Further, the formyl peptide receptor (FPRs) blockade abolished the inhibitory activity of the macrophages treated with the toxin, suggesting the importance of these receptors to the action of CTX on the macrophages antiangiogenic activity. (AU) | |
| FAPESP's process: | 13/17190-7 - Effect of crotoxin on the modulating action of macrophages on angiogenesis: in vitro assays |
| Grantee: | Luciana de Araújo Pimenta |
| Support Opportunities: | Scholarships in Brazil - Master |