Effect of ß-adrenergic sympathetic autonomic nervous system on GLUT4 gene expression on fasting.

Author(s)

Ana Barbara Teixeira Alves

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2007-07-24

Examining board members

Ubiratan Fabres Machado; Luciana Venturini Rossoni; Maria Teresa Zanella

• Advisor: Ubiratan Fabres Machado

Abstract

The study investigated the participation of ß-adrenergic sympathetic nervous system in the regulation of GLUT4 gene expression in red (soleus) and white (EDL) muscles during fasting. Rats were fasted for 48hours and treated with ß1/ß2-adrenoceptors antagonist or ß1/ß2/ß3-adrenoceptors antagonist or ß1/ß2/ß3-adrenoceptors agonist. Soleus and EDL muscles were analyzed by Northern and Western Blotting. Fasting decreased body weight, glycemia, insulinemia, and increased non esterified free fatty acids (NEFA). The insulinemia increased with ß1/ß2 blockade, glycemia decreased with triple blockade, NEFA increased with ß stimulus. The GLUT4 mRNA increased in soleus and EDL after fasting, and it decreased in soleus and EDL after ß1/ß2 blockade and triple blockade, and increased in soleus after ß stimulus. GLUT4 protein increased in soleus of fasted rats, and decreased with triple blockade in EDL. Fasting increased GLUT4 gene expresion in skeletal muscle. The ß-adrenergic sympathetic activity is essencial to increase GLUT4 expression in soleus and to preserve it in EDL. (AU)