Experimental infection with Neospora caninum in yo... - BV FAPESP
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Experimental infection with Neospora caninum in young dogs (Canis familiaris), adults and in pregnant bitches.

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Author(s):
Guacyara Tenorio Cavalcante
Total Authors: 1
Document type: Doctoral Thesis
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:
Examining board members:
Solange Maria Gennari; Katia Denise Saraiva Bresciani; Alda Maria Backx Noronha Madeira; Vera Letticie de Azevedo Ruiz; Teresinha Tizu Sato Schumaker
Advisor: Solange Maria Gennari
Abstract

The objectives of this study were to evaluate transplacental transmission of N. caninum in different stages of pregnancy (Exp I) and evaluate different bovine tis.sues as means of transmission of N. caninum in young dogs and adults (Exp II). In Exp I, Three dogs were inoculated with 108 tachyzoites of N. caninum in the third week of gestation, three at 6 sixth week and one remained as a control. All infected dogs, and at least one of their offspring, seroconverted to anti-N. caninum antibodies by Immunofluorescence Assay. There was presence of the parasite by Hematoxylin- Eosine exam in Central Nervous System and by PCR and RFLP ITS-1 in lymph node, brain, heart and liver. In Exp II, young and adult dogs received different tissues of cattle naturally infected with N. caninum: heart, brain, liver and masseter. None seroconverted. Only the young dogs shed oocysts of N. caninum by eating masseter (2 dogs, 40%), heart (2 dogs, 40%), liver (one dog, 33%) and brain (3 dogs, 75%). (AU)