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| Author(s): |
Guilherme Shigueto Vilar Higa
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2017-11-16 |
| Examining board members: |
Alexandre Hiroaki Kihara;
Luiz Roberto Giorgetti de Britto;
Marcela Bermudez Echeverry;
Cecilia Cerqueira Café Mendes;
Angela Cristina do Valle
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| Advisor: | Alexandre Hiroaki Kihara |
| Abstract | |
Gap junctions (GJ) composed of neuronal connexins (Cx) play a significant role in the activity-dependent circuitry maturation promoting modulation of coherent spontaneous neuronal activity. Herein, we evaluate two major hippocampal neuronal Cxs, Cx36 and Cx45 during postnatal development. We identified changes in Cx36 and Cx45 transcript and protein levels during these developmental periods. Interestingly, immunofluorescence analyses showed that Cx36 and Cx45 are located in all hippocampal subregions. Also, neuronal Cx distribution in these sub-regions is modulated throughout postnatal development. Using electrophysiological recording, we identified changes imposed by GJ blocker over the hippocampal activity in neonates and two-week-old rats. Our finds demonstrate a regulation of neuronal Cxs and distinct GJ role in activity modulation during postnatal hippocampal development, which might be essential to physiological processes that govern proper hippocampal development. (AU) | |
| FAPESP's process: | 12/03583-4 - Identification and functional characterization of connexins in the developmental hippocampus |
| Grantee: | Guilherme Shigueto Vilar Higa |
| Support Opportunities: | Scholarships in Brazil - Doctorate |