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| Author(s): |
Bianca Rodrigues da Cunha
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB) |
| Defense date: | 2017-05-12 |
| Examining board members: |
Eloiza Helena Tajara da Silva;
Marcos Brasilino de Carvalho;
Raquel Ajub Moysés
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| Advisor: | Eloiza Helena Tajara da Silva |
| Abstract | |
Over the past years, it has become evident that cancer initiation and progression depends on several components of the tumor microenvironment, including inflammatory and immune cells, fibroblasts, endothelial cells, adipocytes, and extracellular matrix. These components and the neoplastic cells interact with each other providing pro and antitumor signals. The present study aimed to analyze the secretome of cancer cells under stress and their effect on the tumor microenvironment. For this purpose, two cell lines from head and neck carcinomas were cultured in two stress conditions - hypoxia and radiation. The medium conditioned by these cells (secretome 1) and their control were used to grow untreated neoplastic cells and normal human fibroblasts from oral cavity. Our results showed that signals derived from cancer cells in response to stress drive gene and protein expression and cell behavior. Thirty-eight overexpressed cellular and 9 secreted proteins, and 61 underexpressed cellular and 70 secreted proteins were identified in neoplastic cells under hypoxic stress. Fifty-nine overexpressed cellular and 29 secreted proteins, and 59 underexpressed cellular and 19 secreted proteins were identified in neoplastic cells and normal human fibroblasts treated with the medium conditioned by cells under hypoxic stress. The secretome of cells under stress was not able to induce proliferation of cancer cells and normal human fibroblasts, but promoted migration and invasion. The results may contribute to understand the effect of paracrine factors released by neoplastic cell on gene expression as well as on stromal and tumor cells behavior (AU) | |
| FAPESP's process: | 12/06048-2 - Analysis of the secretome of head and neck carcinoma and its effect on the tumor microenvironment |
| Grantee: | Bianca Rodrigues da Cunha |
| Support Opportunities: | Scholarships in Brazil - Doctorate |