Full text
| |
| Author(s): |
Maria Lair Sabóia de Oliveira Lima
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Química |
| Defense date: | 2013-02-25 |
| Examining board members: |
Anita Jocelyne Marsaioli;
Andre Luiz Meleiro Porto;
Cláudio Francisco Tormena
|
| Advisor: | Anita Jocelyne Marsaioli |
| Abstract | |
Biocatalysts are important in academia and industry joining the ecological friendly to the ecological safe and efficient processes. Thus, the search for new synthetic routes and reactions producing less hazardous residues fits into the principles of green chemistry as environment and residues are taken into consideration. Based on these principles, present study aims at the enzymatic profiling of microorganisms (156 strains) from cooper mine (Sossego¿s mine, Canaã dos Carajás- PA), belonging to VALE. After isolation (228 microorganisms) and preservation, the strains were characterized by mass spectrometry (MALDI-TOF, 62 strains), being characterized bacterias Pseudomonas sp., Bacilus sp., Acinetobacter sp., Delfitia sp. and Escherichia sp.. The enzymatic activities were evaluated by a high throughput screening using fluorogenic probes based on umbelliferone. Outstanding enzymatic activities of these 156 micro-organisms were: esterases (70), lipases (80), epoxy hydrolases (11) and monooxygenases (53). Additionally the implementation of a novel Quick-E methodology with chiral fluorogenic probes and a competitor will allow the rapid evaluation of esterases enantioselectivities in whole cells by measuring inicial rates of bioreactions in micro scale reactions (AU) | |
| FAPESP's process: | 11/10385-1 - Identification of proteins related to process of bioleaching of copper mines |
| Grantee: | Maria Lair Sabóia de Oliveira Lima |
| Support Opportunities: | Scholarships in Brazil - Master |