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| Author(s): |
Jaqueline Poleto Bragato
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Araçatuba. 2021-08-20. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária. Araçatuba |
| Defense date: | 2021-08-06 |
| Advisor: | Valéria Marçal Felix de Lima |
| Abstract | |
Visceral Leishmaniasis (VL) in humans is a chronic and often fatal disease if left untreated. According to World Health Organization, the disease is expanding with a high mortality rate. The Araçatuba/SP region concentrating a large number of cases in the state. Canine Leishmaniasis (CanL) is a serious public health problem because infected animals are powerful transmitters of the parasite to humans by phlebotomine vector, canine disease is more prevalent than human disease and usually canine cases precede human cases. Dogs are therefore an important target in the control measures of the disease. The progression of canine infection is accompanied by failure of cellular immunity with reduction of circulating lymphocytes and cytokines that suppress the function of macrophages. The function of T cell in the induction of cellular response is determinant for the elimination of the parasite inside the macrophages. Although immune suppression is already characterized, the determining factors are poorly understood. In the last decade, studies have shown that the regulation of the effector function of macrophages and T cells seems to depend on miRNAs. There are many evidences of the role of miRNAs in regulating the expression of proteins that are critical for the development, function, and differentiation of various cellular types of the immune system. miRNA-21 has increased expression in splenic leukocytes of dogs with CanL and targets mRNA of several proteins related to the immune response. Our objective was to evaluate the expression of FAS, FASL, CD69, CCR7 and IL-10 proteins in splenic leukocytes after transfection using molecular tools to increase or decrease miR-21. Our results will improve understanding of the pathogenesis of CanL in the host, and as such, reveal new pathways that may be therapeutic targets. (AU) | |
| FAPESP's process: | 18/16239-6 - MiRNA 21 in the regulation of immune response in Canine Visceral Leishmaniasis |
| Grantee: | Jaqueline Poleto Bragato |
| Support Opportunities: | Scholarships in Brazil - Doctorate |