The influence of the supplementation of Omega 3 and melatonin on tissue response of different endodontic sealers

Author(s)

Pedro Henrique Chaves de Oliveira

• Document type: Master's Dissertation
• Press: Araçatuba. 2020-08-17.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba
• Defense date: 2020-07-20
• Advisor: Luciano Tavares Angelo Cintra

Abstract

The aim of this study was to evaluate the influence of omega-3 and melatonin supplements on the tissue response of endodontic sealers. 72 Wistar rats were allocated in 3 main groups: control rats treated with water (TW), rats treated with omega-3 (TO) and rats treated with melatonin (TM). In each rat, 4 polyethylene tubes were implanted, 1 empty tube for control, and the others containing one of the endodontic sealers: Sealapex, AH Plus and Endofil. The evaluation periods were of 5, 15 and 30 days (n = 8 / time). After euthanasia, the tubes were removed and processed for microscopic analysis. Inflammation was evaluated in H.E. staining and immunohistochemical technique for IL-6 and TNF-α, collagen by Picrosírios red staining and calcium deposition by von Kossa and polarized light technique. Statistics were performed according to each type of analysis, with a significance level of 5% (p <0.05) and considering each type of supplementation compared to the control. It is possible to observe a reduced inflammatory infiltrate over time and less intensity in the TO or TM groups compared to TW, as well as the immunolabeling for IL-6 and TNF-α. Sealapex sealer induced less inflammation at 30 days, both in TO and TM. Regarding collagen, a greater expression of immature fibers was observed in the TO group at 15 and 30 days, however, in the TM group, a greater amount of mature fibers was observed at 5 days, both compared to TW. Sealapex sealer was the only one to present positive marking for calcium and birefringent calcite crystals under polarized light, regardless of supplementation. It can be concluded that supplementation with omega-3 or melatonin modulated the inflammation, reducing the inflammatory infiltrate and the immunolabeling of IL-6 and TNF-α, they stimulated tissue repair and did not influence the calcium deposition process. (AU)

• FAPESP's process: 17/24713-7 - Influence of omega-3 and melatonin supplementation on the tissue response of endodontic sealers
• Grantee: Pedro Henrique Chaves de Oliveira
• Support Opportunities: Scholarships in Brazil - Master