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| Author(s): |
Isabela dos Reis Souza
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Araraquara. 2021-07-20. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara |
| Defense date: | 2021-07-08 |
| Advisor: | Carlos Alberto de Souza Costa |
| Abstract | |
Based upon ethical questions and because of the difficulty of getting intact human teeth, researchers have employed bovine teeth to assess some physic and mechanic properties of diverse dental materials. However, lack of scientific data about using such animal teeth model for biological tests of products recommended to be applied on different teeth substrates still remains. Therefore, the aim of this study was to evaluate and compare the transdentinal cytotoxicity of different resin luting cements as well as to use an in vitro model that mimic clinical situations to determine whether bovine dentin (BD) has potential to replace human dentin (HD) for biological tests of indirect toxicity. For this purpose, standardized HD and BD discs (0.4 mm thick) were adapted in artificial pulp chamber devices (APCs), which were individually placed in wells of 24- well plates. Then, odontoblast-like MDPC-23 cells were seeded on the pulpal surface of the dentin discs. After 48- hour incubation, the luting cements were applied on the occlusal surface of the discs, giving rise to the following groups: G1- No treatment on HD (negative control); G2- No treatment on BD (negative control); G3- Single Bond Universal on HD (positive control); G4- Single Bond Universal on BD (positive control); G5- RelyX Luting 2 on HD; G6- RelyX Luting 2 on BD; G7- RelyX U200 on HD; G8- RelyX U200 on BD; G9- RelyX Ultimate on HD; and G10- RelyX Ultimate on BD. MDPC-23 cells as well as human dental pulp cells (HDPCs) were also seeded in wells of 96-well plates. After 24 hours incubation, the transdentinal toxicity on the MDPC-23 cells seeded at the pulp side of the discs was immediately determined by the analysis of cell viability (AlamarBlue assay) and morphology (SEM). The extracts (culture medium + components released from the dental materials that diffused through the dentin discs) were collected and applied on the MDPC-23 cells and HDPCs seeded in wells. Then, these cells were assessed concerning their viability (Live/Dead assay), adhesion and spreading (F-actin), as well as the phenotypic expression of alkaline phosphatase (ALP, thymolphthalein monophosphate) and mineralized nodules formation (Alizarin Red). MN). The numerical data were submitted to ANOVA one-way statistical tests, complemented by Tukey (significance level of 5%). Lower viability of the MDPC-23 cells seeded on dentin was observed after using Single Bond Universal (G3 and G4) and RelyX Luting 2 (G5 e G6) in comparison with their respective negative controls (G1 and G2, p<0.05). There was no significant difference between each dental material when they were applied on HD or BD (p>0.05). The viability of those MDPC23 cells seeded in wells, as well as their adhesion, spreading and ALP activity was significantly lower in G3, G4, G5, G6, G9 and G10 than in G1 and G2 (p<0.05). However, G7 and G8 were not different from the controls G1 and G2 (p>0.05). The viability, adhesion, spreading and ALP activity of the HPDCs was significantly lower in G5 and G6 than in G1 and G2 (p<0.05). Overall, the MDPC-23 cells were more sensitive to the extracts obtained from the dental materials assessed in this study than the HDPCs. No statistical difference occurred when the same dental material was applied on HD or BD (p>0.05). According to the methodology used and the results obtained in this laboratory investigation, one may conclude that only RelyX U200 luting cement does not cause transdentinal toxic effects to pulp cells and that bovine dentin can be employed to replace human dentin for assessing the indirect cytotoxicity of resin-based luting cements (AU) | |
| FAPESP's process: | 19/05788-1 - Influence of human and bovine dentin substrate in the cytotoxicity of materials used for cementation of indirect restorations |
| Grantee: | Isabela dos Reis Souza |
| Support Opportunities: | Scholarships in Brazil - Master |