Evaluation of the alcoholic extract of the bark and almond from Dipteryx alata Vogel (Leguminosae) in the wound healing in mice (lineage C57BL6)

Author(s)

Marcelo Candido Portilho Gouveia

• Document type: Master's Dissertation
• Press: Jaboticabal. 2020-08-27.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
• Defense date: 2020-06-26
• Advisor: Bruno Watanabe Minto

Abstract

Dipteryx alata Vogel (Baru) is one of the native species of the Brazilian cerrado with potential nutritional and pharmacological features. Our study aimed to evaluate the effect of the Baru on wound healing in mice of the C57BL6 lineage, through histological and immunohistochemical analyzes. A 9 mm skin defect was created in the dorsal region of the chest. The animals were divided into 6 groups: Group 01 (n= 9 males) and 02 (n= 9 females) in which the animals received only base cream (vehicle); group 03 (n= 9 males) and 04 (n= 9 females) which received bark extract in 10%; and animals from group 05 (n= 9 males) and 06 (n= 9 females) which received 10% seed extract. All wounds were treated topically twice a day until complete healing, and evaluated at 7, 14 and 21 days postoperatively. There was no statistical difference between groups considering CD31 and picrosirius type III markers. Subtle differences were noted on wound closure velocity, quality of re-epithelialization, neovascularization and collagenization, according to macroscopy and H&E. Additionally, exacerbated inflammation, tissue retraction, loss of hair epithelium or infection were not observed. Dipteryx alata proved to be viable, although no significant favoring for wound healing process has been demonstrated. (AU)

• FAPESP's process: 18/25061-6 - Evaluation of Wound healing in mice treated with alcoholic extract from Dipterix alata Vogel (Leguminosae)
• Grantee: Marcelo Candido Portilho Gouveia
• Support Opportunities: Scholarships in Brazil - Master