Genetic characterization and different culture conditions in Agaricus subrufescens

Author(s)

Wagner Gonçalves Vieira Junior

• Document type: Master's Dissertation
• Press: Jaboticabal. 2021-03-18.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
• Defense date: 2021-02-18
• Advisor: Diego Cunha Zied

Abstract

The species Agaricus subrufescens, popularly known as the sun mushroom, is cultivated in several places around the world, but still on a small scale, compared to the production of other mushrooms. The species is originally from Brazil and, up to the present time, it is produced through adaptations of technologies from the cultivation of other species, consequently the species has mostly a low yield and a long period of cultivation. Thus, this work addresses which are the main processes that influence the agronomic behavior (productivity, number and mass of mushrooms, time for the first flow and precocity) of the final product. For this, the influence of different strains was evaluated, starting from a field cultivation (low technological level) to a climatic chamber cultivation, specific for the production of mushrooms (high technological level), which in both situations the product evaluated was the mushroom. The genetic characterization of the strains was also carried out through the polymorphism rate of the ITS region. In this work, ITS strains type A (ABL 03/44 and ABL 16/01) and type A / B (ABL 04/49, ABL 98/11, ABL 18/01, ABL 19/01 and ABL CS7) were found, which is a characteristic of strains found in the Americas and Europe. Most strains did not show a polymorphism rate. The strain ABL CS7 when cultivated in the field showed productivity (20.95%) close to that found in the climatic chamber, however, of the seven months studied, only two were considered efficient (May and June). The main limitations are unfavorable environmental conditions. The cultivation in climatic chamber showed higher yields, with the ABL 19/01 strain being the most productive with 21.69%. Cultivation in climatic chambers can allow up to 4 cycles to be conducted during the year, due to all conditions being controlled. In both crops, the choice of lineage is essential for high yields. (AU)

• FAPESP's process: 19/00419-8 - Characterization of biomolecules of Agaricus subrufescens in different culture conditions
• Grantee: Wagner Goncalves Vieira Junior
• Support Opportunities: Scholarships in Brazil - Master