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| Author(s): |
Amanda Haisi
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Botucatu. 2021-08-05. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu |
| Defense date: | 2021-06-09 |
| Advisor: | João Pessoa Araújo Junior |
| Abstract | |
The Zika virus belongs to the genus Flavivirus, family Flaviviridae, similar to other arboviruses of public health interest. Its discovery occurred in 1947 with the isolation in captive Rhesus monkey (Macaca mulatta), sentinel in the control of yellow fever at the Uganda forest. Due to the limited knowledge about the role of neotropical primates in ZIKV transmission cycles, the aim of this work was to obtain evidence of enzootic circulation of ZIKV in NHPs from three Brazilian states: São Paulo, Paraíba and Paraná, especially at the interface between urban environment and forest areas. Tissue samples (spleen, heart, liver, lung, kidney and brain) (n=75) were collected post mortem from 34 NHP carcasses from São Paulo and Paraíba state, and blood (n=66) and serum samples (n=58) were obtained from 66 NHP visibility health from Foz do Iguaçu-PR, totaling 100 neotropical primates. All tissue and blood samples (n=141) were tested using a RT-qPCR TaqMan® targeting the envelope gene (E). Serum samples were subjected to detection of anti-ZIKV IgG antibodies and neutralizing antibodies by a commercial indirect ELISA and plaque reduction neutralization test (PRNT), respectively. All samples (n=141) tested negative in the RT-qPCR TaqMan® asay, suggesting the absence of active infection in 100 NHPs collected. Similarly, all serum (n=58) were negative in serological assays for ZIKV, demonstrating the absence of previous viral exposure of captive and free-ranging NHPs from Foz do Iguaçu-PR. In conclusion, no evidences of viral circulation were found in the NHPs during the analyzed period. These results, even if negative, considerably contribute to the epidemiology and surveillance of ZIKV in sylvatic populations. (AU) | |
| FAPESP's process: | 19/05288-9 - Molecular detection and phylogenetic analysis of zika virus (ZIKV) in non-human primates (PNH) in the State of São Paulo and Paraná, Brazil |
| Grantee: | Amanda Haisi |
| Support Opportunities: | Scholarships in Brazil - Master |