Effects of Epstein-Barr virus RPMS1 expression on human cells cultured in vitro

Author(s)

Beatrice Adrianne Silva Jorge

• Document type: Master's Dissertation
• Press: Botucatu. 2020-12-11.
• Institution: Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu
• Defense date: 2020-11-26
• Advisor: Deilson Elgui de Oliveira

Abstract

The Epstein-Barr virus (EBV) – formally human gammaherpesvirus 4 (HHV-4) – is a herpes virus associated with the development of a variety of human malignancies, notably Burkitt lymphoma (BL) and nasopharyngeal carcinoma (NPC), but also subsets of cases for Hodgkin and non-Hodgkin lymphomas (particularly in immunocompromised patients) and gastric carcinomas. RPMS1 is an EBV viral product generated by splicing of the BamHI A transcribed segment (BART) and with only a few studies published about. It is known that this viral protein is involved in the modulation of the Notch pathway and contributes to EBV-induced cell transformation. Of note, a single nucleotide polymorphism (SNP) A / G at position 155391 in the ORF of RPMS1 was associated with an increased risk of NPC. Thus, this work hypothesized that the expression of EBV RPMS1 could influence the phenotype of immortalized and neoplastic human cell lines in vitro. For this, a lentiviral vector containing the ORF of RPMS1 was assembled for future experiments. Due to transduction problems, a vector with constitutional expression of RPMS1 was used to evaluate NP69 cells for cell migration and viability. It was observed that cells that expressed a higher rate of RPMS1 mRNA had lower cell viability. As for the migration, no changes were noticed. In addition, positive EBV lymphoid cells were genotyped for SNP 155391 and all presented the G variant. Finally, the level of RPMS1 expression by lymphoid cells was analyzed, showing that each cell line expresses this gene in different rates. (AU)

• FAPESP's process: 18/12164-1 - Effects of Epstein-Barr protein RPMS1 expression in human cells cultured in vitro
• Grantee: Beatrice Adrianne Silva Jorge
• Support Opportunities: Scholarships in Brazil - Master