Effect of red wine consumption on the induced apical periodontitis development in rats.

Author(s)

Renan Dal Fabbro

• Document type: Doctoral Thesis
• Press: Araçatuba. 2021-08-04.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba
• Defense date: 2021-08-03
• Advisor: João Eduardo Gomes Filho

Abstract

Aim: To evaluate the effect of red wine consumption or its polyphenols on the inflammation/resorption processes associated with periapical periodontitis (PP) in rats. Methodology: Thirty-two 3-month-old Wistar rats had the PP induced in the four first molars, arranged into four groups: control (C) - rats with PP; wine (W) - rats with PP receiving 4.28 mL/kg of red wine; resveratrol+quercetin (R+Q) - rats with PP receiving 4.28 mL/kg of solution containing 1.00 mg/L of quercetin and 0.86 mg/L of resveratrol; and alcohol (ALC) - rats with PP receiving the alcoholic dose contained in the wine. The oral gavage treatments were daily administered, from day 0 to day 45th. At the 15th day the PP was induced, and at the 45th day the animals were euthanized; histological, immunohistochemical (RANKL, OPG, TRAP, IL-10, TNF-⍺ and IL-1β), and micro-computed tomography analysis were performed in the jaws. The Kruskal-Wallis with Dunn's test was performed for nonparametric data, and the ANOVA with Tukey's test for parametric data, p<0.05. Results: The median score of inflammatory process was statistically lower in the R+Q group (1) compared to the C (2) and ALC (3) groups, and not different from the W (1.5) group. Although the W and R+Q groups had a lower score for RANKL, TNF-⍺ and IL-1β, no differences were found compared to C and ALC. The immunolabelling for OPG was higher in the R+Q group compared to all groups; the same observed for IL-10, being different from groups C and ALC. The R+Q group had the lowest TRAP cell count, followed by the W group, both inferior to C and ALC groups, which presented the worst results. The lowest bone resorption value was in the R+Q group (0.50mm3±0.21mm3), statically lower than the C group (0.88mm3±0.10mm3). The W group (0.60 mm3±0.25 mm3) and R+Q group showed less bone resorption compared to the ALC group (0.97mm3±0.22mm3). Conclusion: Red wine administration led to lowers PP inflammation, TRAP marking, and periapical bone resorption compared to ALC; resveratrol-quercetin administration reduced the PP inflammatory processes, periapical bone resorption, and altered the OPG, IL-10, and TRAP expression compared to C and ALC groups. (AU)

• FAPESP's process: 17/27219-3 - Effect of red wine consumption on the induced Apical Periodontitis development in rats
• Grantee: Renan Dal Fabbro
• Support Opportunities: Scholarships in Brazil - Doctorate