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| Author(s): |
Barbara Lepretti de Nadai
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Carlos. |
| Institution: | Universidade de São Paulo (USP). Escola de Engenharia de São Carlos (EESC/SBD) |
| Defense date: | 2021-09-03 |
| Examining board members: |
Juliano Jose Corbi;
Flavia Virginio Fonseca;
Guilherme Rossi Gorni;
Lincoln Suesdek Rocha;
Marcel Okamoto Tanaka
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| Advisor: | Juliano Jose Corbi; Gustavo Enrique de Almeida Prado Alves Batista |
| Abstract | |
The Aedes aegypti mosquito is a vector of devastating diseases that can result in severe damage or even death, such as dengue, chikungunya, Zika, and yellow fever. The high adaptability of Ae. aegypti makes the control of this mosquito a significant challenge for public health agencies. Effective mosquito control requires efficient monitoring systems to evaluate results. Monitoring can also provide knowledge about the spatio-temporal distribution of insects, risk assessment, and guidelines for mosquito-borne disease prevention programs. In this sense, new technological devices are being studied to monitor mosquitoes by automatically identifying and counting the species in real-time. One of them is the optical sensor, which can capture mosquitoes wingbeat frequency (WBF) This sensor can be attached to traps to monitor adult mosquitoes\' by the WBF. However, for better accuracy of these sensors, it is necessary to know the environmental conditions that can alter the WBF of mosquitoes. Thus, this research aims to study in detail the WBF of Ae. aegypti mosquitoes, using an optical sensor, in several simulated environmental conditions in the laboratory. The conditions tested were: absence/scarcity of food during the larval phase, different mosquito body sizes, mosquitoes reared in water with different pH values, different air temperatures, humidities, and adults\' age. The results showed that the two mosquito body sizes tested influenced the WBF of the females in an inverse correlation. Small females beat their wings about 40 Hz faster than large ones. Small males, on the other hand, beat their wings about 40 Hz slower than large males. The pH of the larval rearing water impacted the WBF of adults and the wing centroid size of males and females. PH also influenced the weight of females. The temperature affected the WBF of mosquitoes, in a positive correlation. At 28 and 33ºC of temperature, females beat their wings about 56 Hz and 111 Hz faster than females at a lower temperature (23ºC ) respectively. Males at 28 and 33ºC of temperature beat their wings about 98 Hz and 170 Hz faster than males at 23ºC, respectively. Humidity influenced the WBF of almost all groups of mosquitoes tested, except for small males at 20ºC. The wingbeat frequency was also impacted by mosquito age, being lower in the first three days after adult emergence. These results highlight the relevance of studying the environmental conditions that can affect the WBF of mosquitoes. These results may aid in improving the accuracy of optical devices that use WBF for automatic species identification and improve adults mosquitoes monitoring, and aid in understanding the ecology and biology of mosquitoes. (AU) | |
| FAPESP's process: | 16/24622-9 - Study of mosquito Aedes aegypti frequency wings beat using intelligent sensors: simulation of different environmental conditions in laboratory |
| Grantee: | Barbara Lepretti de Nadai |
| Support Opportunities: | Scholarships in Brazil - Doctorate |