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Genetic variation in three groups of individuals (adults, younths and seedlings) of Calophyllum brasiliense Camb. (Clusiaceae), in gallery forest

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Author(s):
Cristina Borges Kawaguici
Total Authors: 1
Document type: Master's Dissertation
Press: Piracicaba.
Institution: Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC)
Defense date:
Advisor: Paulo Yoshio Kageyama
Abstract

With intention of investigating the genetic variability of the usual species, with environmental restriction, it was studied a Calophyllum brasiliense population, species that just happens in areas where water saturation of the soil exists, in three groups of individuais (adults, youths and seedlings). The studied population is placed in the forest of gallery of the ecological Station of Panga, distributed along the river of the Panga, that constitutes the north limit of the reservation. Starting from the analysis of isozyme electrophoresis, 30 adult individuais were studied, 30 yuong and 100 seedlings of Calophyllum brasiliense. It was obtained 22 alleles distributed in 14 loci enzymatic, and 8 were monomorphic and 6 polymorphyc. The frequencies allelics was aleatory, what didn't allow interfervencein the occurrence of selection in the individual group for another. The Wright's F among the groups evidenced low genetic divergence between seedlings and youths (0,0356), seedlings and adults (0,0543) and yuongs and adults, that indicates little drift effects and or selection. The dear diversity indexes for population revealed low alleles number for loci (Aseedlins= 1, 71; Ayoung= 1,57 and Aadults= 1,64 ). The percentage or polymorphic loci was P= 42,86%, that value is commonly similar to the values found to the rare species. The expected medi um heterozigosity was more large than that observed in all the groups, the values observed medium heterozigosity are respectively for seedelings, youths and adults 0,191; 0,138 and 0,141, the more part of genetic variability meets inside of the seedlings group. The fixation index was positive for seedlings,youths and adults, this value was more pronounced in the adults, it's can be occur decause the study area have been colonized recently or then, the adult individuais are more adapted to the establishment in flooded areas, this way, it would be having seedlings selection for the individuals estabilishment, to favour homozygotes. The estimates of adherencefor the Hardy-Weinberg balance didn't evidence differences among the groups, some loci are in balance, others donts. The results of effective size, demonstrated that how many Iarge the endogamic, small is the value of the effective size. That estimate also intervence in the possible strategies of conservation genetics in situ, seeking recovery of degraded areas. (AU)