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| Author(s): |
Wesley José dos Santos
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Botucatu. 2016-07-22. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina. Botucatu |
| Defense date: | 2016-07-15 |
| Advisor: | Simone Baldini Lucheis; Virgínia Bodelão Richini Pereira |
| Abstract | |
Wild animals, both free-living and the captive ones, can be reservoirs and carriers of various protozoa, such as members of the genus Trypanosoma. Trypanosoma cruzi, the etiologic agent of Chagas disease, is one of the most known trypanosomes. Chagas disease is manifested clinically with a multifaceted character, affecting humans and several species of wild and domestic animals, such as non-human primates, which can be reservoirs of this parasite. Regarding the Neo-tropical primates, different infections occur by trypanosomes that also can affect humans. In this study, 39 blood samples were collected from nonhuman primates from Zoo Park Municipal of Bauru, São Paulo, Brazil, by serological and molecular analysis for T. cruzi infection using specific primers for kDNA region (TCZ1 and TCZ2) and the region encoding the heat shock protein (hsp70). All animals were negative to the serological test for anti-T. cruzi and molecular for T. cruzi with primers for kDNA region. However, with the use of primers for hsp70, 11 of 39 (28.2%) animals were positive. The sequencing results showed similarity to the genus Trypanosoma. Despite the difficulties in stablish the species of trypanosome among these primates, it is concluded that triatomine vectors and/or bloodsucking flies may be present in the animal environment; so, the entomologic vigilance for these insects are highly recommended. (AU) | |
| FAPESP's process: | 14/12186-4 - Trypanosoma cruzi in non-human primates from Bauru Zoo – SP |
| Grantee: | Wesley José dos Santos |
| Support Opportunities: | Scholarships in Brazil - Master |