Evaluation of the effects of inflammation on the respiratory infection caused by Streptococcus pneumoniae in mice.

Author(s)

Rubia Isler Mancuso

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2016-06-21

Examining board members

Maria Leonor Sarno de Oliveira; Rita de Cassia Cafe Ferreira; Roxane Maria Fontes Piazza

• Advisor: Maria Leonor Sarno de Oliveira; Orlando Garcia Ribeiro Filho

Abstract

Acute inflammatory response is an important defense against Streptococcus pneumoniae, but persistence of inflammation may result in tissue damage. The susceptibility against an invasive respiratory pneumococcal challenge was evaluated in two outbred mice strains, genetically selected for maximum (AIRmax) and minimum (AIRmin) acute inflammatory responses. The challenge induced the death of 100% of the AIRmin mice and only 36.4% of the AIRmax mice. Characterization of the innate immune responses showed that both mice strains responded to the challenge with the secretion of inflammatory cytokines. However, only the AIRmax mice controlled the inflammation. Significant differences on the expression of matrix metalloproteinases suggest a role of these proteins in the control of the infection. Moreover, the AIRmin mice presented an increase in the number of macrophages expressing the CD206 mannose receptor after the challenge. A reduced resistance of macrophages and neutrophils from AIRmin mice to programmed cell death, after the challenge, was also observed. (AU)

• FAPESP's process: 13/26052-7 - Evaluation of the effects of inflammation on the respiratory infection caused by Streptococcus pneumoniae in mice.
• Grantee: Rubia Isler Mancuso
• Support Opportunities: Scholarships in Brazil - Master