Effect of Methyl Vanillate and Methyl Divanillate on breast cancer cells: regulation of the NADPH oxidase complex

Author(s)

Adriano de Souza Pessôa

• Document type: Master's Dissertation
• Press: Bauru.
• Institution: Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB)
• Defense date: 2020-07-24

Examining board members

Rodrigo Cardoso de Oliveira; Carla Andreotti Damante; Ana Paula Favaro Trombone Garlet; Willian Fernando Zambuzzi

• Advisor: Rodrigo Cardoso de Oliveira; Valdecir Farias Ximenes

Abstract

Breast cancer is the most common cancer among women. The search for new strategies for less invasive treatments and harmful to the body are widely studied. In this context, phytochemicals and their derivatives are known to exhibit a wide range of beneficial biochemical and pharmacological activities. This study evaluated the cytotoxic and anticancer effects of Methyl Vanillate (MV) and Methyl Divanillate (DMV) compounds on MCF-7 and MDA-MB-231 cancer cell lines, estrogen dependent and triple negative, respectively. Firstly, the following concentrations 250 to 4000 M of MV; 25 to 400 M of DMV on HB4a (control line), MCF-7 and MDA-MB-231 cell lines were tested by MTT assay. Then, the cells were treated with 780 M of the VM and 100 M of the DMV after 72h to evaluate the anticancer efficiency . The cells were stained using Crystal Violet to evaluate of viability. The morphology of cell was verified by Hematoxylin/Eosin and DAPI/Phalloidin stains. The number of apoptotic cells were quantified by flow cytometry. The proliferation of cells was analyzed by flow cytometry. The ROS production was performed using DCFH-da by fluorescence. NOX gene expression was evaluated by RT-qPCR. Data were analyzed by one-way ANOVA. Significant differences between groups were determined by Dunnett\'s post-hoc test at p < 0.05. DMV decreased the viability cell, showing higher apoptotic effect and inhibition of proliferation cell compared to VM, on both cell lines. The compounds have shown pro-oxidant action and did not regulate the NADPH oxidase complex. The dimer showed more effectivity and pro-oxidant effect on cell line MCF-7 then MDA-MB-231 using this experimental model. (AU)

• FAPESP's process: 17/26309-9 - Effect of methyl Vanillate and methyl divanilate on breast cancer cells: regulation of the NADPH oxidase complex
• Grantee: Adriano de Souza Pessoa
• Support Opportunities: Scholarships in Brazil - Master