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Morphological, cytogenetic and molecular characterization of Mazama rondoni Miranda-Ribeiro, 1914

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Author(s):
Gabrielle Queiroz Vacari
Total Authors: 1
Document type: Master's Dissertation
Press: Jaboticabal. 2021-11-16.
Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
Defense date:
Advisor: José Mauricio Barbanti Duarte
Abstract

The Mazama rondoni species has been placed as synonymy of Mazama nemorivaga. It is considered part of the gray brocket group that occupies the Amazon region. The taxonomic classification of M. nemorivaga has demonstrated inconsistencies suggesting the presence of more than one species within the taxon. Thus, the present study aimed to contribute to the taxonomy of the genus Mazama by collecting a specimen of M. rondoni in its type locality. It was performed a detail morphological, cytogenetic, and molecular characterization for comparison with other specimens of Mazama in order to evaluate the differences between them and review the taxonomy of the species. For this purpose, we extracted and amplificated mitochondrial DNA from the skull and skin tissue samples of the type series (holotype and paratypes) that were deposited in the National Museum of Rio de Janeiro. We also collected one specimen from the type locality of M. rondoni (Vilhena-RO, Brazil) with the support of local hunters. The topotype was characterized by traditional morphological techniques (craniomandibular measurements, body biometry, and skin color), cytogenetic (conventional Giemsa staining, C band, G band, Ag-NOR and FISH), and molecular (phylogenetic analysis of mitochondrial genes). Our phylogenetic tree recovered the M. rondoni topotype with other individuals of M. nemorivaga from the western Amazon region (Rondônia). We believe that our results contributes as the first step to clarify the identity of the taxon and possible validation of the Mazama rondoni species. (AU)

FAPESP's process: 19/05420-4 - Morphological, cytogenetic and molecular characterization of Mazama rondoni (Miranda Ribeiro, 1914)
Grantee:Gabrielle Queiroz Vacari
Support Opportunities: Scholarships in Brazil - Master