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| Author(s): |
Juliana Souza Uzeloto
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Presidente Prudente. 2020-11-16. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências e Tecnologia. Presidente Prudente |
| Defense date: | 2020-10-30 |
| Advisor: | Ercy Mara Cipulo Ramos |
| Abstract | |
Chronic Obstructive Pulmonary Disease (COPD), in addition to pulmonary impairment, has systemic manifestations that contribute to the worsening of the disease, especially systemic inflammation. Biomarkers are used to investigate the diseases pathophysiology and progression as well as to uncover responses to interventions. In particular, analysis of intracellular cytokine expression presents itself as a more robust option for the evaluation of inflammatory biomarkers, as it allows the specific cell population investigation. Among the treatment options for COPD patients, physical exercise stands out for its great functional benefits and quality of life. However, it is unclear about the relationship between inflammatory biomarkers and clinical and functional characteristics and moreover, there is no knowledge about the effect of physical exercise on intracellular cytokines. Objectives: To evaluate the intracellular cytokines expression, to investigate the correlation between these inflammatory biomarkers and clinical and functional characteristics, and also to evaluate the effect of physical exercise on systemic intracellular inflammation in patients with stable COPD. Methods: COPD patients were evaluated in several clinical aspects (symptoms, quality of life, vital signs and body composition), functional (pulmonary function, level of physical activity, peripheral muscle strength and exercise functional capacity) and intracellular systemic inflammation (IL-8, IL-13, IL-17, IL-6, IL-2, IL-10 and TNF-α in CD4 + T lymphocytes). Inflammation was also investigated after an eight-week intervention, based on physical exercise. Results: 140 patients were recruited, 36 patients in a single moment and 23 patients after the proposed intervention were analyzed. The main findings of this thesis were that, in cross sectional analysis, patients with more severe COPD, in relation to lung function, have greater expression of CD4+ IL-2+. In addition, there was a negative correlation between IL-8+ and peripheral oxygen saturation and steps per day and a positive correlation between IL-10 + and muscle strength of knee flexors and extensors. Additionally, there was a reduction in IL-8 and an increase in IL-13 and TNFα in CD4+ T lymphocytes after eight weeks of physical exercise in patients with stable COPD. Conclusion: Based on the findings of the studies presented, it was possible to deepen the knowledge on different aspects of inflammatory biomarkers of patients with stable COPD and also the effect of physical exercise on these systemic inflammation immunomodulators. (AU) | |
| FAPESP's process: | 18/04870-3 - INVESTIGATION OF THE EFFECT OF COMBINED PHYSICAL TRAINING ON THE INFLAMMATORY PROFILE AND FUNCTIONAL CAPACITY OF COPD PATIENTS: RANDOMIZED CLINICAL STUDY |
| Grantee: | Juliana Souza Uzeloto |
| Support Opportunities: | Scholarships in Brazil - Doctorate |